Shehata, R., Zareh, E., Moustafa, E., Elkhyat, M., Makhlof, N. (2023). Impact of Treatment of Chronic HCV Patients by Direct Acting Antiviral Drugs (DAADs) on COVID -19 Disease Frequency and Severity. Afro-Egyptian Journal of Infectious and Endemic Diseases, 13(4), 225-239. doi: 10.21608/aeji.2023.210472.1290
Rasha H Shehata; Eman A Zareh; Ehab F Moustafa; Mariam Elkhyat; Nahed A Makhlof. "Impact of Treatment of Chronic HCV Patients by Direct Acting Antiviral Drugs (DAADs) on COVID -19 Disease Frequency and Severity". Afro-Egyptian Journal of Infectious and Endemic Diseases, 13, 4, 2023, 225-239. doi: 10.21608/aeji.2023.210472.1290
Shehata, R., Zareh, E., Moustafa, E., Elkhyat, M., Makhlof, N. (2023). 'Impact of Treatment of Chronic HCV Patients by Direct Acting Antiviral Drugs (DAADs) on COVID -19 Disease Frequency and Severity', Afro-Egyptian Journal of Infectious and Endemic Diseases, 13(4), pp. 225-239. doi: 10.21608/aeji.2023.210472.1290
Shehata, R., Zareh, E., Moustafa, E., Elkhyat, M., Makhlof, N. Impact of Treatment of Chronic HCV Patients by Direct Acting Antiviral Drugs (DAADs) on COVID -19 Disease Frequency and Severity. Afro-Egyptian Journal of Infectious and Endemic Diseases, 2023; 13(4): 225-239. doi: 10.21608/aeji.2023.210472.1290
Impact of Treatment of Chronic HCV Patients by Direct Acting Antiviral Drugs (DAADs) on COVID -19 Disease Frequency and Severity
1Tropical Medicine and Gastroenterology Department, Faculty of Medicine, Assiut University, Assiut, Egypt
2Public Health and Community Medicine Department, Faculty of Medicine, Assiut University, Assiut, Egypt
Abstract
Background and study aim: Since both SARS-CoV-2 and Hepatitis C virus (HCV) are positive-sense RNA viruses, it is logical to consider those nucleotide analogues found to be successful against HCV could potentially demonstrate similar efficacy against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The present study aims at estimating the frequency and severity of COVID-19 in HCV-treated patients. Patients and Methods: Cross-sectional study was accomplished. The study enrolled 200 HCV-infected individuals who were eligible for DAADs therapy and had received treatment in 2019 before the COVID-19 pandemic. The treatment regimen for all patients in the study consisted of Sofosbuvir plus Daclatasvir for a duration of 3 months, except for three patients who received the same treatment for 6 months as a result of liver cirrhosis. Results: The enrolled patients attained mean age of 53.6 years. Most patients (74%) came from rural areas, and forty-seven (23.5%) patients had different chronic diseases, with hypertension (17.5%) and diabetes mellitus (13%) being the most common. The majority, 183 (91.5%), of patients achieved sustained virological response (SVR). Only 12 (6%) patients developed COVID-19. In most patients (75%), the duration of infection till seroconversion was 10 days. All COVID-19 patients in the current study, were ultimately improved with no mortality. Nine (75%) of SARS-CoV-2 infected patients developed infection one year after the last dose of DAADs. Most of those patients rarely follow the recommended hygienic measures. Conclusion and recommendations: Previous treatment of chronic hepatitis C by Sofosbuvir plus Daclatasvir provides protection against infection of SARS-CoV-2. Future large, randomized trials are warranted to draw firm conclusions.
Highlights
- There is still a debate about the gold standard therapy for COVID-19 infection
- Patients who were previously treated with antivirals(DAADs) for chronic hepatitis C virus infection may be protected form COVID-19.
- Future studies are still warranted to draw firm conclusion
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has had a profound impact on the globe, with millions of people around the globe affected by the resulting coronavirus disease (COVID-19). The virus has caused several reported deaths and ongoing morbidity, making it a major global health challenge [1].
Both hepatitis C virus (HCV) and coronaviruses are positive-sense single-stranded RNA viruses, which depend uponan RNA-dependent RNA polymerase (RdRp) for genome replication and transcription[2, 3].
Therefore, RdRp is considered a critical target for successful antiviral treatment due to its high conservation at the amino acid level in the active site. In vitro studies have shown that sofosbuvir is capable of binding to SARS-CoV-2 RdRp, which results in a reduction in the protein's function and ultimately leads to the eradication of the virus [4].
Antiviral agents that act by inhibiting polymerases through nucleotide and nucleoside analogs are a potential class of therapeutic agents for treating COVID-19 [4, 5]. Sofosbuvir is an antiviral drug that has been affirmed for clinical use and has shown to be highly effective against different genotypes of the Hepatitis C Virus (HCV) [6]. The daclatasvir and sofosbuvir safety has been established in patients experiencing significantly impaired renal function [7].
In silico and in vitro studies have provided indication that designates the binding of sofosbuvir/daclatasvir and ribavirin to the RNA-dependent RNA polymerase of SARS-CoV-2 [8-10].
Sofosbuvir and daclatasvir have the potential to be accessible and cost-effective treatment agents for COVID-19, considering their availability and affordability. Some authors were persuaded to conduct a clinical trial to appraise the consequence of direct acting antiviral drugs (DAADs) such as Sofosbuvir, Daclatasvir, and Ribavirin on COVID-19. Published data showed challenging findings for combining Daclatasvir and Sofosbuvir in treating COVID-19 patients [11].
The present work intended to assess the COVID-19 severity and prevalence in HCV patients. Also, to judge the consequence of the previous treatment of HCV patients by (DAADs) on COVID-19 infection.
PATIENTS AND METHODS
Study setting and design:
A cross-sectional study was undertaken at Al-Rajhi Liver Hospital. It was conducted in the period between July 2021 and April 2022.
Study population:
The study enrolled 200 individuals with known chronic HCV infections who were eligible for the DAADs in 2019.
Inclusion criteria:
1) HCV patient with or without LC.
2) Age >18 years old.
3) Received the DAADs in 2019.
Exclusion criteria:
Patients who experienced any of the following events were excluded; combined HCV with HIV or HBV infection, age ≤ 18 years old, breast-feeding or pregnant patients, autoimmune liver disease, multiorgan failure, active cancer, and/or renal insufficiency, immunosuppressive drugs and/or confirmed diagnosis of SARS-CoV-2 before starting DAADs.
Methodology
All patients were assisted using a questionnaire with 4 domains (supplementary table 1):
1- Personal and demographic data, socioeconomic status. and other comorbid diseases.
2- Drug therapy for HCV, its duration, type of response, and the period between the end of the last dose of HCV treatment and the emergence of symptoms of COVID-19.
3- Adherence of the patients to precautions against SARS-COV-2.
4- SARS-CoV-2 infection:
• The definition of SARS-CoV-2 infection used in the study adhered to the suspected and confirmed case definitions provided by the Ministry of Health and Population.
• Onset, course, duration, symptoms (Respiratory or GIT or others), hospital admission history, and need for oxygen therapy or ICU admission.
• Clinical outcomes: time of hospital discharge or time of recovery, requirement for invasive mechanical ventilation, and/or death if occurred.
The following investigation was obtained from each patient.
a) Laboratory:
Complete blood count.
Liver enzymes.
PCR for HCV, C-Reactive Protein, ESR, LDH, serum ferritin.
Nasopharyngeal Swab for SARS-COV-2 results if were done.
b) Imaging included chest X-Ray and computed tomography if needed.
Supplementary Table 1
Questionnaire for Chronic HCV treated patients to detect COVID-19
Patient name:
Age:
Social Status:
Married
Single
Divorced
Widow
Gender:
Male
Female
Residence:
Urban
Rural
Occupation:
What is the highest academic qualification ?
Do you smoke ?
Yes
No
Do you have any chronic disease ?
Yes
No
What is the usual daily medications ?
DM ttt
HTN ttt
Chest disease ttt
Cardiac ttt
Others
Do you have current HCV symptoms ?
No
Sore muscle
Joint pain
Fever
Nausea or poor appetite
Itching
Jaundice or dark urine
Do you have Liver cirrhosis ?
Yes
No
What about virological response after ttt ?
SVR
Failure of response
Relapse
NO PCR had been done
Regimen of therapy of HCV and it's duration?
Sofosbuvir and Daclatasvir 3 months.
Sofosbuvir and Daclatasvir 6 months.
Sofosbuvir, Daclatasvir, and ribavirin 3 months.
Sofosbuvir, Daclatasvir, and ribavirin 6 months.
What is the time period between the end of the last dose of DAADs and the emergence of symptoms of Corona virus if happens ?
3-6 months
6-9 months
9-12 months
>1 year
No COVID-19 infection happened
In the last months, Did you attend social gathering outside your home ?
Most days (>8days)
Some days (4-7days)
Few days (<3days)
Not had any other social interaction
Did you wear facial mask in your daily activity ?
Always
Usually
Rare
Never
Others
In the past month, have you been in contact with a person known to have been diagnosed COVID-19 ?
Yes
No
How often are you doing the recommended pandemic hygiene, like washing your hands frequently, avoiding touching your face, covering coughs, avoiding frequently touched surfaces in public places?
All the time, I am being extra careful.
Most of the time. I am trying to do my best.
Sometimes, I do it if I remember it.
Rarely, I don't worry about thes things.
How often have you used public transport ?
Most days (>8 days).
Some days (4-7 days).
Few days (< 3 days).
Not using public transport.
Did you have COVID-19 ?
Yes (confirmed)
Yes (but suspected)
No
During the past months, did you have an illness that you think might have been COVID-19, the novel Corona virus ?
Yes
No
When did the illness Start ?
Have you been told by a physician or healthcare provider that you have had covid 19 ?
Yes
No
Were you tested for COVID-19 by PCR ?
Yes
No
What is the result of PCR test of Corona virus ?
Positive
Negative
Not done
Which symptoms did you have during the illness ?
Fever, chills/ shivering
Shortness of breath /increased
Newly developed Cough /increased
Sore throat
Funny nose
Nausea, vomiting, diarrhea
Generalized body ache, fatigue
Headache
Chest pain
New Inability to taste or smell
No symptoms
Others
Have you had radiological evidence of pneumonia chest X ray or MSCT ?
Yes
No
Not done
Were you admitted to the hospital ?
Yes
No
Were you cared for in ICU ?
Yes
No
How many days did you stay at hospital ?
No hospital admission
7 days or less
7-14 days
14-30 days
More than 1 month
Did you need to have a breathing tube or ventilator ?
Yes
No
How long was the period of infection with COVID-19 ?
How long did it take to get a negative test?
> 10 days
≤ 10 days
No PCR had been done
Mention drug regimen that had been taken during COVID-19 19 infection ?
Panadol
Vitamin C
Zinc
Antibiotic
Antiviral
Clexan
Corticosteroid
Lactoferrine
Chloroquine
Is there clinical improvement and no limitation of movement after COVID-19 infection ?
Yes
No
Did you need ventilation or high flow oxygen therapy ?
Yes
No
Did you need intubation and mechanical ventilation ?
Yes
No
What is the fate of the patient after COVID-19 infection?
Survival
Death
Laboratory investigation that have been done during the Illness
I. Liver enzymes
Normal
Elevated
II. Inflamatory markers
LDH
Normal
Elevated
ESR
Normal
Elevated
CRP
Normal
Elevated
S. ferritin
Normal
Elevated
III. CBC
Hemoglobin level
Normal
Decreased
Lymphopenia
Present
Absent
Platelet count
Normal
Decreased
Statistical analysis:
Data analysis was accomplished by SPSS software package version 21 (SPSS inc. chicago. illinois. USA). Continuous data were presented as mean and SD, while nominal data were presented as frequencies (percentages). A Chi2 test was carried out to compare the occurrence of COVID-19 infection and other characteristics between the two patient groups. The statistical significance of the P value was determined at a confidence level of 95%, indicating that a value below 0.05 would be considered significant for the study's purposes.
RESULTS
Demographic characteristics of the studied patients (Table 1):
The age of the patients engaged in this study ranged from 20 to 88 years, with a mean age of 53.6 years. Out of those patients, 105 (52.5%) patients were males.
Thirty- eight (19%) patients were smokers.
Drug therapy for HCV, its duration, and response (Table 2):
Only three patients in this study had liver cirrhosis, and those patients received Sofosbuvir with daclatasvir for 6 months, while the majority (98.5%) of patients had chronic hepatitis and received the same regimen for 3 months. Most patients, 183 (91.5%), attained sustained virological response (SVR).
Adherence of the patients to precautions against SARS CoV-2 (Table 3):
Thirty-seven (18.5%) patients never attended social gatherings outside the home, while 51 (25.5%), 38 (19%), and 74 (37%) patients attended such activities either a few days, some days, or most days, respectively. Eighty-seven (43.5%) patients never wear facial masks, while 35 (17.5%) patients usually wear them, and only 12 (6%) patients always wear them. There were 48 (24%) patients with a history of contact with confirmed COVID-19 cases.
Frequency of confirmed COVID-19 infection in the studied patients (Figure 1):
Twelve (out of 200 patients)(6%) had been confirmed to have COVID-19 infection.
Clinical, laboratory data, and outcome of confirmed COVID-19 cases among the studied patients (Table 4):
All patients had a fever, while the other most frequent symptoms were loss of taste and smell (83.3%), cough (66.7%), and dyspnea (50%). In the majority of COVID-19 patients (75%), COVID-19 was diagnosed after one year from the last dose of DAADs. In only 3 patients, COVID-19 was diagnosed within one year from last DAADs dose. All patients with COVID-19 in this study were completely improved and survived.
Demographic data, Comorbidity, HCV treatment, and adherences to protective measures among patients who developed COVID-19 in our study (Table 5):
The mean age of those patients was 55.6 years, the majority (66.7%) were females, and 10 (83.3%) patients came from rural areas. Fifty percent had a chronic disease, mainly HTN (41.7%) and DM (33.3%).
Fifty percent attended social gatherings outside the home, and 8 (66.7%) patients never wear facial masks in daily activities. There were 7 (58.3%) patients who had a history of contact with a positive case of SARS-CoV-2 infection. Most patients (50%) rarely follow the recommended hygienic measures.
Comparison of patients'characteristics based on the occurrence of COVID-19 (Table 6):
Both groups based on the occurrence of COVID-19 had insignificant differences as regards different characteristics and adherence to the hygienic measures (p> 0.05) except for the significantly higher frequency of contact with infect patients (58.3% vs. 21.8%; p< 0.001) and HTN (41.7% vs. 16%; p= 0.04) among patients with COVID-19 infection.
Table (1): Demographic characteristics of the studied patients
Variable
N= 200
Age (years)
Range
53.6 ± 15.3
20-88
Sex
Male
Female
105 (52.5%)
95 (47.5%)
Residence
Rural
Urban
148 (74%)
52 (26%)
Social status
Single
Married
Divorced
Widow
11 (5.5%)
175 (87.5%)
2 (1%)
12 (6%)
Comorbidities (Yes )*
47 (23.5%)
Hypertension
35 (17.5%)
Diabetes mellitus
26 (13%)
Cardiac disease
5 (2.5%)
Chest disease
3 (1.5%)
Smoking
38 (19%)
Education level
Illiterate
Primary
Secondary
University/postgraduate
124 (62%)
4 (2%)
31 (15.5%)
41 (20.5%)
Occupation
Farmer
Worker
Employee
23 (11.5%)
129 (64.5%)
48 (24%)
Data were expressed as frequency (percentage), mean (SD), range
*Some patients had more than one comorbid chronic disease.
Table (2): Drug therapy for HCV, its duration and response
Variable
N= 200
Liver cirrhosis
3 (1.5%)
Drug regimens
Sofosbuvir/daclatasvir for 3 months
Sofosbuvir/daclatasvir for 6 months
197 (98.5%)
3 (1.5%)
Sustained virological response
Yes
No
183 (91.5%)
17 (8.5%)
Data were expressed as frequency (percentage). HCV: hepatitis C virus
SVR: sustained virological response
Table (3): Adherence of the patients to precautions against SARS CoV-2
Variable
N= 200
Attendance social gathering
No
Few days (< 3 days)
Some days (4-7 days)
Most days (> 8 days)
37 (18.5%)
51 (25.5%)
38 (19%)
74 (37%)
Wear facial mask in the daily activity
Never
Rare
Usually
Always
87 (43.5%)
66 (33%)
35 (17.5%)
12 (6%)
Contact with infected patients
48 (24%)
How frequency did you follow the recommended hygienic measures
Rarely (I don’t worry about these things)
Sometimes (I do it if I remember it)
Most of the time (I’m trying to do my best)
All the time (I’m being extra careful)
67 (33.5%)
90 (45%)
31 (15.5%)
12 (6%)
Usage of public transport
No
Few days (< 3 days)
Some days (4-7 days)
Most days (> 8 days)
113 (56.5%)
41 (20.5%)
20 (10%)
26 (13%)
Data were expressed as frequency (percentage). SARS CoV-2:
Severe acute respiratory syndrome coronavirus 2.
Table (4): Clinical, laboratory data, and outcome of confirmed COVID-19 cases among the studied patients
Variable
N= 12
Clinical manifestations
Fever
Loss of taste and smell
Cough
Dyspnea
Fatigue
Headache
Nausea/vomiting
Diarrhea
12 (100%)
10 (83.3%)
8 (66.7%)
6 (50%)
4 (33.3%)
3 (25%)
2 (16.7%)
1 (8.3%)
Duration from the last DAADs dose to the emergence of COVID-19 symptoms
3-6 months
7-9 months
10-12 months
> 12 months
1 (8.3%)
1 (8.3%)
1 (8.3%)
9 (75%)
Pneumonia
3 (25%)
Hospital admission
1 (8.3%)
Hospital stays (days)
< 7
Oxygen therapy
1 (8.3%)
Raised CRP
1 (8.3%)
Raised ESR
1 (8.3%)
Normal hemoglobin
12 (100%)
Normal platelets
12 (100%)
Normal liver enzymes
12 (100%)
Normal ferritin
12 (100%)
Normal LDH
12 (100%)
Duration till seroconversion
7 days
10 days
15 days
1 (8.3%)
9 (75%)
2 (16.7%)
Survival
12 (100%)
Data were expressed as frequency (percentage). COVID-19: coronavirus -19 disease; DAAs: direct acting antiviral agents; CRP: c-reactive protein; ESR: erythrocyte sedimentation rate; LDH: lactate dehydrogenase.
Table (5): Demographic data, Comorbidity, HCV treatment and adherences to protective measures among patients who developed COVID-19 in our study
Variable
N= 12
Age (years)
55.6 ± 15.3
Sex
Male
Female
4 (33.3%)
8 (66.7%)
Residence
Rural
Urban
10 (83.3%)
2 (16.7%)
Married
12 (100%)
Hypertension
5 (41.7%)
Diabetes mellitus
4 (33.3%)
Cardiac disease
1 (8.3%)
Chest disease
1 (8.3%)
Smoking
1 (8.3%)
Education level
Illiterate
Secondary
University/postgraduate
8 (66.7%)
3 (25%)
1 (8.3%)
Occupation
Farmer
Employee
10 (83.3%)
2 (16.7%)
Drug regimens
Sofosbuvir/daclatasvir for 3 months
12 (100%)
Sustained virological response
Yes
No
10 (83.3%)
2 (16.7%)
Attendance of social gathering
None
Some days (4-7 days)
Most days (> 8 days)
4 (33.3%)
1 (16.7%)
6 (50%)
Wear facial mask in daily activity
Never
Rare
8 (66.7%)
4 (33.3%)
Contact with infected patients
7 (58.3%)
Follow the recommended hygienic measures
Rarely (I don’t worry about these things)
Sometimes (I do it if I remember it)
Most of the time (I’m trying to do my best)
6 (50%)
4 (33.3%)
2 (16.7%)
Usage of public transport
None
Few days (< 3 days)
Some days (4-7 days)
7 (58.3%)
3 (25%)
2 (16.7%)
Data were expressed as frequency (percentage), mean (SD). COVID-19: coronavirus infectious disease-19.
Table (6): Comparison of patients' characteristics based on the occurrence of COVID-19 *
Variable
Occurrence of COVID-19
P value
Yes (n= 12)
No (n= 188)
Gender
Male
Female
4 (33.3%)
8 (66.7%)
101 (53.7%)
87 (46.3%)
0.14
Diabetes mellitus
4 (33.3%)
22 (11.7%)
0.06
Hypertension
5 (41.7%)
30 (16%)
0.04
Cardiac disease
1 (8.3%)
4 (2.1%)
0.26
Chest disease
1 (8.3%)
2 (1.1%)
0.17
Residence
Rural
Urban
10 (83.3%)
2 (16.7%)
138 (73.4%)
50 (26.6%)
0.35
Education level
Illiterate
Primary
Secondary
University/postgraduate
8 (66.7%)
0
3 (25%)
1 (8.3%)
116 (61.7%)
4 (2.1%)
28 (14.9%)
40 (21.3%)
0.58
Attendance social gathering
None
Few days (< 3 days)
Some days (4-7 days)
Most days (> 8 days
4 (33.3%)
1 (8.3%)
1 (8.3%)
6 (50%)
33 (17.6%)
50 (26.6%)
37 (19.7%)
68 (36.2%)
0.27
Wear facial mask in daily activity
Never
Rare
Usually
Always
8 (66.7%)
4 (33.3%)
0
0
79 (42%)
62 (33%)
35 (18.6%)
12 (6.4%)
0.20
Contact with infected patients
7 (58.3%)
41 (21.8%)
< 0.001
How frequency did you follow the recommended hygienic measures
Rarely
Sometimes
Most of the time
All the time
6 (50%)
4 (33.3%)
2 (16.7%)
0
61 (32.4%)
86 (45.7%)
29 (15.4%)
12 (6.4%)
0.53
Usage of public transport
None
Few days (< 3 days)
Some days (4-7 days)
Most days (> 8 days)
7 (58.3%)
3 (25%)
1 (8.3%)
1 (8.3%)
106 (56.4%)
38 (20.2%)
19 (10.1%)
25 (13.3%)
0.94
Date was expressed as frequency (percentage). P value was significant if < 0.05.
* The Chi-square statistic test (Chi2) was carried out to compare patients' characteristics between the two patient groups.
DISCUSSION
The replicating process of SARS-CoV-2, a positive-sense RNA virus, is highly dependent on an RdRp. Sofosbuvir plus Daclatasvir has been shown to inhibit HCV replication effectively and can inhibit the replication of SARS-CoV-2. Since other viral families exhibit a similar replication mechanism, there is a possibility of using a specific antiviral regimen interchangeably, particularly during the SARS-CoV-2 pandemic [12].
In this context, we aimed to judge the influence of treating chronic HCV patients by direct acting antiviral drugs (DAADs) on COVID-19 disease frequency and severity. Two hundred patients who confirmed HCV infection and were eligible for therapy with DAADs were involved. All patients received Sofosbuvir plus Daclatasvir for 3 months except three patients who received the same regimen for 6 months due to liver cirrhosis.
The main findings in this study were: 1) the used regimen was effective in the management of HCV, where 91.5% of patients achieved SVR, 2) the majority of studied patients did not strictly follow the precautions against SARS-CoV-2 infection, and 74% were coming from rural areas, 3) only 12 (6%) of patients developed COVID-19 and all of them were alive and 4) nine (75%) of those patients with COVID-19 developed their SARS-CoV-2 infection one year since the last dose of DAADs.
Regarding the efficacy of Daclatasvir and Sofosbuvir for treating chronic hepatitis C virus infection, many studies confirmed this point [13-17]. Nouh et al [17] stated that out of 401 patients diagnosed with chronic HCV infection who received Daclatasvir and Sofosbuvir, 385 (96%) patients achieved SVR.
Although the results have been mixed, preclinical trials specify that Sofosbuvir and Daclatasvir have the potential to act on SARS-CoV-2 RdRp. Multiple clinical studies have suggested that Sofosbuvir and Daclatasvir may have potential therapeutic effects for COVID-19 patients [18-20].
The progress of developing nucleoside analogs against respiratory viruses has been evident for coronavirus and influenza viruses but developing such drugs has been slower for Respiratory Syncytial virus (RSV), adenovirus, and mostly rhinovirus. Due to the acute nature of airway infections caused by these viruses, it is essential to initiate antiviral drug treatment promptly after the onset of symptoms [21].
The FDA has expanded its Remdesivir approval to comprise outpatients who are at risk of developing severe COVID-19, in addition to hospitalized patients. Although nucleoside analogs have the potential to restrict the dispersal of respiratory viruses through the community, their effectiveness for this aim has not been fully established. For broader use, orally administered drugs may be more practical compared to those necessitating inhalation or injection [22].
Recently,a meta-analysis indicated that Sofosbuvir and Daclatasvir usage could lessen the mortality rate and requirement for mechanical ventilation in individuals hospitalized with moderate to severe cases of COVID-19. Patients who received Sofosbuvir and Daclatasvir showed a higher overall clinical recovery rate, particularly among those who were hospitalized [23].
A previous study assessed the effectiveness of adding Sofosbuvir and Daclatasvir to the conventional therapy of 174 patients with confirmed COVID-19 infection. The authors found that adding those two drugs had beneficial effects in shortening the hospital stay and rapid seroconversion but had no effect on mortality [24].
A study found that severe COVID-19 patients who were treated with hydroxychloroquine (HCQ) along with Sofosbuvir and Daclatasvir (35 patients) or ribavirin (27 patients) had significantly different outcomes. Specifically, the group receiving Sofosbuvir and Daclatasvir had a lower mortality rate (6% vs. 33%) and shorter hospitalization duration (5 days vs. 9 days) compared to the group receiving ribavirin [19].
Another study compared the use of Sofosbuvir and Daclatasvir with hydroxychloroquine (HCQ) to HCQ alone in 55 patients. The study found no significant difference in symptom relief on Day 7 between the two groups [12]. Assessing the efficacy of Sofosbuvir and Daclatasvir after only 7 days may not fully reflect their potential benefits, which becomes more obvious after Day 14 [24].
Generally, Sofosbuvir-based treatment was found to be linked to a substantially higher rate of clinical recovery, reduced rate of necessitating mechanical ventilation, and intensive care unit admission. In addition, patients who received this treatment had shorter hospital stays and recovery times compared to those in the control groups. Moreover, Sofosbuvir-based treatment was consistently observed to provide clinical benefits in hospitalized patients with COVID-19, according to subgroup analyses[25]. Furthermore, the subgroup analysis revealed that patients who received Sofosbuvir and Daclatasvir experienced improved clinical outcomes compared to those who received comparators, consistent with previous studies' findings [23, 26].
We noticed that our study is different from those cited studies, where in the majority of those studies, there was another group used as a control to compare the effect of Sofosbuvir/Daclatasvir on the outcome of patients who were already diagnosed to have COVID-19 infection. Nevertheless, our study assessed the frequency of developing COVID1-9 infection following the usage of Sofosbuvir/Daclatasvir in individuals experiencing chronic HCV infection.
In the present work, we discovered that only 12 (6%) HCV patients who received Sofosbuvir/Daclatasvir therapy had confirmed COVID-19 and all of them had mild to moderate disease and were alive. In addition, most of them had their COVID-19 after 1 year from last dose of DAADs.
A retrospective case-control study found that patients who received chronic hepatitis C treatment using Sofosbuvir and Daclatasvir exhibited a reduced SARS-CoV-2 infection rate (2.2%, 11 SARS- CoV- 2 infections) relative to cases of the control group (6%, 30 SARS-CoV-2 infections with significant differences regard the severity [27].
In the comparison of our patients' characteristics based on the occurrence of COVID-19, we found a significantly higher frequency of contact with infected patients (58.3% vs. 21.8%; p< 0.001) and hypertension (41.7% vs. 16%; p= 0.04) among patients with COVID-19 infection versus those without.
Middle-aged individuals are typically infected within the community, whereas older individuals are more susceptible to infections through contact with family members or during hospitalization due to coughing and sneezing from infected individuals [28]. Men appear to be more susceptible to SARS-CoV-2 infection, making the male sex a risk factor for COVID-19. Other risk factors for COVID-19 infection include DM, malignancy, cardiovascular diseases, and other conditions that weaken immunity [29].
Significant risk factors for COVID-19 infection were male gender, age group over 60 years, residing in densely populated areas, being married, having close contact with individuals infected with COVID-19, and underlying health conditions [30].
Limitation of the study: The current study has several limitations, including a small sample size, a single-center study, and the absence of a control group.
CONCLUSION
Previous chronic hepatitis C treatment by Sofosbuvir and Daclatasvir provides protection against SARS-CoV-2 infection. If chronic HCV patients who finished treatment with DAADs had COVID-19, their disease course was mild, and they ultimately improved. Larger randomized controlled studies and multi-center Egyptian studies are necessitated to confirm the effectiveness of direct acting antiviral drugs (DAADs) as a possible SARS-CoV-2 infection therapy.
Competing interest: Not applicable.
Funding: Not applicable.
Ethical considerations: The aim of the study was explained to participants before filling the questionnaire and written informed consent was obtained.
Acknowledgments: Not applicable
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