Interplay Between Tumor Necrosis Factor-α, Insulin Resistance and Type 2 Diabetes Mellitus in Chronic Hepatitis C Egyptian Patients Interplay Between Tumor Necrosis Factor-α, Insulin Resistance and Type 2 Diabetes Mellitus in Chronic Hepatitis C Egyptian Patients

Elkady, Mostafa; Omar, Maha Z; Elbehisy, Mona M; Mohamed, Mohamed A

doi:10.21608/aeji.2015.17845

Interplay Between Tumor Necrosis Factor-α, Insulin Resistance and Type 2 Diabetes Mellitus in Chronic Hepatitis C Egyptian Patients Interplay Between Tumor Necrosis Factor-α, Insulin Resistance and Type 2 Diabetes Mellitus in Chronic Hepatitis C Egyptian Patients

Document Type : Original Article

Authors

¹ Department of Hepatology, Gastroenterology and Infectious Diseases, Benha University,Egypt

² Department of Clinical Pathology, Benha University,Egypt.

³ New General Mansoura Hospital , Egypt

10.21608/aeji.2015.17845

Abstract

Background and study aim : Hepatitis C is a disease with significant global impact, it is the most common cause of chronic liver diseases, and in addition it causes insulin resistance (IR) leading to increase the risk of type 2 diabetes mellitus (DM). This current study aimed to assess the relationship between serum tumor necrosis factor-α (TNF-α), insulin resistance (IR) and type 2 DM in patients HCV.
Patients and Methods: The study cohort consisted of 91 subjects stratified into 4 groups; Group (I): Included 25 HCV patients without DM, Group (II): Included 25 HCV diabetic patients, Group (III): Included 25 diabetic patients without HCV infectionand group (IV): Included 16 healthy subjects serving as a control group. All patients were subjected to full history taking, thorough clinical examination and estimation of body mass index (BMI). Anti-HCV Ab was detected by the 3rd generation (ELISA) testand was confirmed by PCR. Assessment of fasting plasma insulin level (FBI) and TNF-α were done by ELISA test, while assessment of the insulin resistance was estimated by Homeostatic Model Assessment (HOMA-IR).
Results: Higher mean levels of FBS, 2 hr (2HPP) and fasting plasma insulin (FSI) were detected in group II (HCV+DM) compared to other groups with statistically significant differences between all the studied groups (P value <0.001), consequently HCV diabetic patients were found to have significant higher IR than HCV patients without DM, diabetic patients alone and control group (P value <0.001). Furthermore, there was highly statistically significant differences between all studied groups as regard level of TNF-α (P value <0.001) with higher mean level in group I (HCV group). Insignificant difference in level of TNF-α in HCV patients with or without IR (P value =0.072). Insignificant positive correlation between HOMA-IR and TNF-α (P value = 0.63).
Conclusion: Chronic HCV patients have significantly elevated fasting plasma insulin level, TNF-α and significant IR and there was insignificant correlation between HOMA-IR and TNF-α.

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