Association between Cyclin D1 G870A Polymorphism and the Risk of Hepatocellular Carcinoma among Egyptian Population

Elkhashab, Mohamed N; Refaey, Mohamed; Radwan, Mohamed; Pasha, Heba F; Ebian, Hoda F; Makhlouf, Rodalia M; Ibrahim, Mohamed H

doi:10.21608/aeji.2015.17851

Association between Cyclin D1 G870A Polymorphism and the Risk of Hepatocellular Carcinoma among Egyptian Population

Document Type : Original Article

Authors

¹ Tropical Medicine Department, Faculty of Medicine, Zagazig University, Egypt.

² Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt.

³ Clinical Pathology Department, Faculty of Medicine, Zagazig University, Egypt.

⁴ Internal Medicine Department, Faculty of Medicine, Zagazig University, Egypt.

10.21608/aeji.2015.17851

Abstract

Background and study aim : Cyclin is a regulatory protein in cell cycle encoded by CCND1 gene, it has a role in transition of cell cycle from G1 phase to S phase. A common polymorphism is in the exon of splicing donor region G to A polymorphism (G 870A) of cyclin D1 result in two types of mRNA, cycline D1a and D1b. Both may cause disturbance and uncontrolled growth of the cells which may lead to high susceptibility of certain tumor development.
This polymorphism could be detected by using polymerase chain reaction (PCR-RFLP) assay. Our present study was performed to demonstrate the association between G870A polymorphism in (CND1) gene and hepatocellular carcinoma (HCC) among the Egyptian population.
Patients and methods: The present study was consisting of 180 peripheral blood samples from 90 subjects diagnosed with HCV without HCC, 90 subjects diagnosed with HCV and HCC and 90 individual cancer free controls matched on age, gender, smoking and DM status.
Results: Our results determined the high frequency of the alleles in the patients (G, 40.6% and A, 30.0%). Our results determined also the frequencies of the case subjects compared to control subjects (59.4% versus 13.3%) and P value <0.001, also analysis of CCND1 870 gene polymorphism revealed that genotype AA and GA were significantly, more frequent in patients with HCC compared with GG genotypes as a reference among the Egyptian population as we found the odds ratios (ORS) for GA, AA and GG respectively were GG 2.27 (1.1-4.2) P= 0.02, AA 6.82 (1.51-7.8) P= 0.004 and GG OR 1.00 with confidence interval CI: 95%.
Conclusion: Our study results demonstrated that single nucleotide polymorphism of CCND1 G870A among the Egyptian population is associated with increased risk of HCC.

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