Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine DepartmentAfro-Egyptian Journal of Infectious and Endemic Diseases2090-76136320160901Impact of Helicobacter pylori Eradication on Absolute Telomere Length in Gastric Mucosa121127995410.21608/aeji.2016.9954ENMaha . OmarDepartment of Hepatology, Gastroenterology and Infectious Diseases, Faculty of Medicine, Benha University, EgyptAbeer AAboelazmMedical Microbiology and Immunology, Faculty of Medicine, Benha University, EgyptReem RAbd El-GlilMedical Microbiology and Immunology, Faculty of Medicine, Benha University, EgyptJournal Article20180731<strong>Background and study aim: </strong>This study proposed to assess the relation between absolute telomere lengths (TLs) in gastric mucosa and <em>Helicobacter pylori </em>(<em>H. pylori</em>) infection and study the impact of (<em>H. pylori</em>) eradication therapy on TLs.<br /> <strong>Patients and Methods: </strong>This study was conducted on (42) subjects divided into two groups, group I: included (17) <em>H. pylori</em> negative cases served as control group, group II: included (25) <em>H. pylori</em> positive patients. Absolute TLs was measured in base pairs (bp) in gastric mucosa and analyzed by real time polymerase chain reaction (RT-PCR) in all groups and re-evaluated in <em>H. pylori</em> positive patients 4 weeks after eradication therapy.<br /> <strong>Results:</strong> Prior to eradication therapy, there was highly significant shortening (P<0.001) in TLs (bp) in gastric mucosa of <em>H. pylori</em> positive patients compared to <em>H. pylori</em> negative controls. While there was highly significant elongation was observed after <em>H. pylori</em> eradication therapy in H. pylori positive patients (P < 0.001). There was significant negative correlation between TLs and ages of patients (P<0.001) while there was no significant relation between TLs and sex (P= 0.5) before and after eradication therapy.<br /> <strong>Conclusion:</strong> <em>H. pylori</em> positive patients had significantly shorter TLs than <em>H. pylori</em> negative controls. TLs were increased after <em>H. pylori</em> eradication therapy in H. pylori positive cases. This finding may indicate the importance of <em>H. pylori</em>eradication to avoid the development of gastric cancer by its effect on TLs.https://aeji.journals.ekb.eg/article_9954_216936c3cf806aec85ec7077ccac690c.pdfZagazig University, Faculty of Medicine, Endemic and Tropical Medicine DepartmentAfro-Egyptian Journal of Infectious and Endemic Diseases2090-76136320160901Relationship between Plasma Concentrations of Interleukin-10 and Thrombocytopenia in Chickenpox Patients128133995610.21608/aeji.2016.9956ENMariam Al-FadhliDepartment of Medicine, Infectious Disease Hospital, Ministry of Health, KuwaitMohammad AhmedSarayaDepartment of Tropical Medicine, Faculty of Medicine, Zagazig University, Zagazig, Egypt.Journal Article20180731<strong>Background and study aim:</strong> Chickenpox is highly infectious, self-limiting disease, caused by varicella-zoster virus. Thrombocytopenia frequently complicates chickenpox. This study was conducted to assess the correlation between serum interleukin 10 levels and thrombocytopenia in adult chickenpox patients.
<strong>Patients and Methods:</strong> Fifty patients of chickenpox complicated by thrombocytopenia were included in this study as a patient group. Fifty patients of chickenpox with normal platelet of comparable age, race and gender were identified as a control group. All patients were diagnosed by clinical picture of chickenpox and by presence of varicella - zoster virus immunoglobulin M (VZV IgM) in the patient blood. Estimation of complete blood count, kidney function tests, liver function tests, fasting blood sugar, and levels of cytokines IL-10 and TNF-α were done in both groups on the day of admission and day of discharge.
<strong>Results:</strong> At time of admission, IL-10 levels were significantly higher in patients group than in control group, while TNF-α level were not significantly different between the two groups. At time of discharge, we observed a significant decline in IL-10 levels in the patients group as compared with time of admission. A negative correlation between IL-10 levels and platelet count was observed while no correlation was found between TNF-α and platelet count.
<strong>Conclusion:</strong> IL-10 level could play an important role in development of thrombocytopenia in patients with chickenpox.
https://aeji.journals.ekb.eg/article_9956_97bcc0dbccf2597298342252d2169b47.pdfZagazig University, Faculty of Medicine, Endemic and Tropical Medicine DepartmentAfro-Egyptian Journal of Infectious and Endemic Diseases2090-76136320160901Frequency of Cholelithiasis in Patients with Chronic Liver Disease: A Hospital-Based Study134141995810.21608/aeji.2016.9958ENMona AhmedAbdelmaksoudTropical Medicine department, Faculty of Medicine, Zagazig University, EgyptMostafa HEl-ShamyTropical Medicine department, Faculty of Medicine, Zagazig University, EgyptHala IMHusseinTropical Medicine department, Faculty of Medicine, Zagazig University, EgyptBihery SAhmedTropical Medicine department, Faculty of Medicine, Zagazig University, EgyptHussein AhmedMedical Research Group of Egypt ,Faculty of Medicine, Zagazig University, Zagazig, EgyptHoda Abdel-AzizEl-HadyInternal Medicine Department, Faculty of Medicine, Zagazig University, EgyptJournal Article20180731<strong>Background and study aim: </strong>Liver Cirrhosis is a strong and a common known risk factor for Cholelithiasis. Cholelithiasis is a multifactorial disease, based on a complex interaction of environmental and genetic factors. The primary aim of this study is to determine the frequency of cholelithiasis in chronic liver disease (CLD) patients admitted to Zagazig university hospitals. The secondary aim is to determine the risk factors and their association with the underlying etiology and severity of liver disease.<br /> <strong>Patients and Methods:</strong> We conducted a hospital based study including 131 patients with chronic liver disease based on clinical, laboratory and Ultrasonographic findings. Demographic, clinical and etiological data were recorded, using a pre-coded questionnaire. A number of laboratory tests as fasting plasma glucose, total cholesterol, triglyceride, aspartate aminotransferase (AST), alanine amino-transferase (ALT), alkaline phosphatase (ALP), hepatitis B surface antigen (HBsAg), and antibody to hepatitis C virus (HCV-Ab) were analyzed.<br /> <strong>Results:</strong> The number of registered cases was 131 with age (52.9±11.7).There were 55 (42%) males and 76 (58%) females. Hepatitis C (HCV) was present in 101 (77%) cases. The prevalence of cholelithiasis was 50.4%% (66 of 131 patients). Most of cholelithiasis patients presented with child C stage (68.2%), followed by child B (21.2%) and the least one was Child A. Hepatitis C (10.6%) was found to be associated with cholelithiasis (75.8%), followed by hepatitis B (13.6%). Auto-immune disease, diabetes mellitus, contra-ceptive pills and obesity are considered risk factors for cholelithiasis.<br /> <strong>Conclusion:</strong> Cholelithiasis tends to occur more frequently in patients with decompensated CLD. The higher incidence of cholelithiasis in CLD appears to be associated with HCV infection. This is an important parameter to be considered in a country with high prevalence of HCV as Egypt.https://aeji.journals.ekb.eg/article_9958_6698067ac0b19fc581f890873e4d4cd6.pdfZagazig University, Faculty of Medicine, Endemic and Tropical Medicine DepartmentAfro-Egyptian Journal of Infectious and Endemic Diseases2090-76136320160901A molecular Approach for Evaluation of Experimental Trials of Anti Schistosomal Vaccination in Murine Models142151996010.21608/aeji.2016.9960ENSamia EEtewaMedical Parasitology Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.Mohamed HAbdel HadyMedical Parasitology Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.Ashraf SMetwallyMedical Parasitology Departments, Faculty of Medicine
Zagazig University, Zagazig, Egypt.Somia . HAbd AllahBiochemistry Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.Sally MShalabyBiochemistry Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.Amal SEl-ShalBiochemistry Department, Faculty of Medicine
Zagazig University, Zagazig, EgyptMahmoud AEl ShafeyClinical Pathology Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.Howayda S.FMoawadMedical Parasitology Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.Journal Article20180731<strong>Background and study aim:</strong> Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believed that the best long term strategy to control schistosomiasis is through immunization with anti-schistosomiasis vaccines. This study aims at assessment of the efficacy of different potential anti-schistosomal vaccines (as crude soluble egg antigens (SEA), soluble worm antigen preparation (SWAP) and combined SEA & SWAP) by parasitological and molecular studies in experimental murine models.
<strong>Materials and Methods:</strong> Sixty male laboratory bred Swiss Albino mice were used and divided into six groups; control normal (G1), control infected by ± 80 cercariae by S.C. route (G2), Freund’s adjuvant (adj.) received then infected (G3), SEA+adj. received then infected (G4), SWAP+ adj. received then infected (G5) and combined (SEA+SWAP) + adj. received then infected (G6). A schedule of sensitization, immunization and schistosomiasis challenge were followed and performed on different mice groups. Mice were euthanized 10 weeks post-infection. Potential vaccine efficacy was investigated by parasitological and molecular studies including egg count/gram stool using modified Kato thick smear<strong>,</strong>liver egg load, oogram pattern in the liver and<strong> </strong>stool PCR to detect <em>S</em>. <em>mansoni </em>egg DNA in stools of studied mice.
<strong>Results:</strong> The combined (SEA+SWAP) vaccine caused the highest significant reduction in the fecal egg count followed by SWAP then SEA antigens. On the other hand, the highest percentage reduction in eggs/gram liver tissue was attributed to the combined (SEA+SWAP) followed by SEA then SWAP antigens. Regarding oogram results, the combined (SEA+ SWAP) antigens were more efficient in increasing the number of dead ova with highly significant reduction in the number of mature & immature ova, followed by SEA then SWAP antigens.<strong> </strong>The lowest percentage of <em>S. mansoni</em> egg DNA detected by PCR in stool samples<strong> </strong>was encountered with the combined (SEA+ SWAP), followed by SEA then SWAP antigens.
<strong>Conclusion:</strong>The parasitological and PCR-based assessment studies denoted that the combined (SEA+SWAP) vaccine candidate was the most effective in protection against schistosomiasis challenge. The results of parasitological and molecular studies were nearly similar but the molecular study was more sensitive, definite and accurate.https://aeji.journals.ekb.eg/article_9960_5ff7023dd570c829a0ebaac273c9bff3.pdfZagazig University, Faculty of Medicine, Endemic and Tropical Medicine DepartmentAfro-Egyptian Journal of Infectious and Endemic Diseases2090-76136320160901Hydatidosis in Morocco: Review of literature and epidemiology152155996210.21608/aeji.2016.9962ENZineb TlamcaniDepartment of Parasitology, Faculty of Medicine and Pharmacy of Fes,
University of Sidi Mohammed Ben Abdellah ,Fes, Morocco,Journal Article20180731Hydatidosis or hydatid cyst is a prevalent zoonosis all over the world due to larval forms of the tapeworm of the genus Echinococcus. Echinococcus granulosus is the most frequent form of echinococcal infection in humans. The disease generally results from an oral ingestion of the parasite ova eliminated with feces of dogs leading to the emergence of hydatid cysts.<br /> Hydatidosis is a health problem that may remain asymptomatic for several years. This disease is endemic in Morocco and is recorded to be a serious problem that requires great caution.https://aeji.journals.ekb.eg/article_9962_ff1ffb18c0a1b0c0dfa109f35195a8fe.pdf