eng
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
Afro-Egyptian Journal of Infectious and Endemic Diseases
2090-7613
2090-7184
2015-03-01
5
1
1
6
10.21608/aeji.2015.17061
17061
Original Article
Study of Interleukin-18 in Chronic hepatitis C virus related liver diseases
Mohamed Nouh
1
Hatem El-Sebaai
2
Hossam Mohamed
3
Hossam El-Din Seleem
4
Usama Khalil
5
Tropical Medicine Department, Faculty of Medicine, Menofia University, Egypt
Biochemistry Department, Faculty of Medicine, Menofia University, Egypt
Tropical Medicine Department, Faculty of Medicine, Menofia University, Egypt
Tropical Medicine Department, Faculty of Medicine, Menofia University, Egypt
Mansoura Fever Hospital, Mansoura, Egypt
Background and study aim: ELISA can determine serum Interleukin (IL)-18 level. It is a sensitive, simple and rapid test, thus help to study changes of serum IL-18 levels in chronic HCV related liver diseases during different stages. The objective of this study was to study serum IL-18 levels in chronic HCV related liver diseases. Patients and methods: Sera from 60 patients with HCV related chronic liver diseases at various stages of HCV infection (chronic hepatitis, cirrhosis and complications) and sera of 10 normal controls were subjected to measurements of serum IL-18 level by ELISA assay. Results: There were highly significant increase in the mean values of serum IL-18 in chronic HCV related liver cirrhosis, non complicated and complicated patients in comparison to chronic active hepatitis C patients and healthy subjects and highly significant increase in the mean values of serum IL-18 in complicated patients in comparison to non complicated patients. There was highly significant increase in the mean values of serum IL-18 in decompensated liver cirrhosis patients when compared to compensated patients. Conclusion: Serum IL-18 level shows highly positive significant correlation with severity of liver dysfunction in HCV related liver cirrhosis.
https://aeji.journals.ekb.eg/article_17061_b40c0c2012d0d71e7571444609e03ed1.pdf
interleukin
hepatitis C
ELISA
Cirrhosis
eng
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
Afro-Egyptian Journal of Infectious and Endemic Diseases
2090-7613
2090-7184
2015-03-01
5
1
7
14
10.21608/aeji.2015.17062
17062
Original Article
Vitamin D Profile : Can it Affect the Response to Standard Hepatitis C Treatment in Egyptian Patients ?
Mohamed Farghaly
rana4emo90@yahoo.com
1
Hala Husseein
2
Noha Shaheen
3
Nagla Abdel-Wahab
4
Heba Pasha
hebapasha@yahoo.com
5
Tropical Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Tropical Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Tropical Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Tropical Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Background and study aim : Vitamin D is a potent immunomodulator. It is reported to be related to the severity of fibrosis and responsiveness to interferon-based therapy in genotype 1 chronic hepatitis C (CHC), so we aimed to evaluate if there is an association between vitamin D metabolism related genes and vitamin D level with the degree of liver damage and the response to treatment of CHC in our locality. Patients and methods : Two hundred and forty five Egyptian patients (123 patients with sustained virological response and 122 patients with treatment failure) were included. They were subjected to routine investigation needed for treatment, in addition to estimation of 25-OH vitamin D level in serum by ELISA and CYP27B1-1260 gene polymorphism by PCR-RFLP method. Results: We found that serum levels of vitamin D showed statistically significant increase in responders in comparison with non responders. The distribution of CYP27B1-1260 AC and CC genotypes were significantly presented in the non responders in comparison with the responders. CYP27B1-1260 AC and CC genotypes carriers had a high risk for treatment failure, (OR= 14.7, 95% CI= 2.3-20.1, P<0.001; OR = 20.4, 95% CI= 2.9-21.3, P Conclusion: Vitamin D serum level and CYB27B1 -1260 genotype could be used as a predictor to anti HCV treatment response in our locality.
https://aeji.journals.ekb.eg/article_17062_6b4e90f88766b284c8c10d1334080fe9.pdf
vitamen D
HCV
Treatment
Egyptian
eng
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
Afro-Egyptian Journal of Infectious and Endemic Diseases
2090-7613
2090-7184
2015-03-19
5
1
15
23
10.21608/aeji.2015.17177
17177
Original Article
Screening for Opportunistic Intestinal Parasites in HIV/AIDS Patients, Attending the Services of Medical Care in Three Different Hospitals, Southern Ethiopia
Feleke Eriso
feleke.eriso@yahoo.com
1
Department of Biology, College of Natural and Computational Sciences, Dilla University, Dilla, Ethiopia
Background and study aim: Diagnostic examination of stools for opportunistic intestinal parasites in HIV/AIDS patients is given less attention than it should be. The suspected opportunistic intestinal parasites such as Cryptosporidium parvum,Cyclospora cayentanensis, Toxoplasma gondii, Isospora belli and the symptom of explosive watery diarrhea they cause as well as others including Strongyloides stercoralis are the threat against the well-being of HIV/AIDS patients. The objective of this study is to demonstrate the indispensable necessity to free HIV/AIDS patients (who are under medical care in 3 different hospitals, Southern Ethiopia), from opportunistic intestinal parasites using diagnostic examination of stools followed by prompt curative treatment during every safety time interval. Patients and methods: Fresh stools samples from a total sample size of 710 HIV/AIDS patients were taken and examined in the parasitology laboratory, Dilla University, for the suspected intestinal opportunistic parasites. The methods employed to identify the intestinal parasites included observations in : wet mount, formalin-ether concentration technique, and permanent slide preparation as well as Baermann apparatus method for Strongyloides stercoralis. Result: Out of 710 HIV/AIDS patients examined 196 were found to be positive for 6 different species of the suspected intestinal parasites (infection rate of these parasites in the population of HIV/AIDS patients of the 3 different hospitals being 196×100/710=27.6% The six species of parasites isolated from fresh stools samples were: Ascaris lumbricoides, Strongyloides stercoralis, Entameba histolytica, Giardia lamblia, Balantidium coli, and Trichuris trichiura. Conclusion: Reasonably planned successive safety time intervals must be attended continuously by HIV/AIDS patients without interruption to utilize the services of medical care in order to avoid/ neutralize the potential opportunistic infections and reinfections; otherwise, the fulminant death can turn to be true. The safety and well-being of those HIV-infected patients who attend all the medical services & advices provided by clinical experts is not different from that of HIV-noninfected individuals without any trace of exaggeration.
https://aeji.journals.ekb.eg/article_17177_4e8aaa50b467bb1070b9b65b2cce1789.pdf
HIV/AIDS
hospital
morbidity
opportunistic
mortality
patients
Parasites
eng
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
Afro-Egyptian Journal of Infectious and Endemic Diseases
2090-7613
2090-7184
2015-03-19
5
1
24
32
10.21608/aeji.2015.17178
17178
Original Article
Study of Acid-Base Disturbances in Patients with Liver Cirrhosis
Mohamed Nouh
1
Hossam Mohamed
2
Basam Masoud
3
Abd El-Fattah Yassin
4
Tropical Medicine Department, Faculty of Medicine, Menoufia University, Menoufia, Egypt
Tropical Medicine Department, Faculty of Medicine, Menoufia University, Menoufia, Egypt
Tropical Medicine Department, Faculty of Medicine, Menoufia University, Menoufia, Egypt
Mansoura Fever Hospital, Mansoura Egypt
Background and study aim: Diagnostic examination of stools for opportunistic intestinal parasites in HIV/AIDS patients is given less attention than it should be. The suspected opportunistic intestinal parasites such as Cryptosporidium parvum,Cyclospora cayentanensis, Toxoplasma gondii, Isospora belli and the symptom of explosive watery diarrhea they cause as well as others including Strongyloides stercoralis are the threat against the well-being of HIV/AIDS patients. The objective of this study is to demonstrate the indispensable necessity to free HIV/AIDS patients (who are under medical care in 3 different hospitals, Southern Ethiopia), from opportunistic intestinal parasites using diagnostic examination of stools followed by prompt curative treatment during every safety time interval. Patients and methods: Fresh stools samples from a total sample size of 710 HIV/AIDS patients were taken and examined in the parasitology laboratory, Dilla University, for the suspected intestinal opportunistic parasites. The methods employed to identify the intestinal parasites included observations in : wet mount, formalin-ether concentration technique, and permanent slide preparation as well as Baermann apparatus method for Strongyloides stercoralis. Result: Out of 710 HIV/AIDS patients examined 196 were found to be positive for 6 different species of the suspected intestinal parasites (infection rate of these parasites in the population of HIV/AIDS patients of the 3 different hospitals being 196×100/710=27.6% The six species of parasites isolated from fresh stools samples were: Ascaris lumbricoides, Strongyloides stercoralis, Entameba histolytica, Giardia lamblia, Balantidium coli, and Trichuris trichiura. Conclusion: Reasonably planned successive safety time intervals must be attended continuously by HIV/AIDS patients without interruption to utilize the services of medical care in order to avoid/ neutralize the potential opportunistic infections and reinfections; otherwise, the fulminant death can turn to be true. The safety and well-being of those HIV-infected patients who attend all the medical services & advices provided by clinical experts is not different from that of HIV-noninfected individuals without any trace of exaggeration.
https://aeji.journals.ekb.eg/article_17178_e9ef73a9be4d9fdb75c4d32b15d760a6.pdf
Liver cirrhosis
acid base balance disorders
respiratory alkalosis
eng
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
Afro-Egyptian Journal of Infectious and Endemic Diseases
2090-7613
2090-7184
2015-03-19
5
1
33
39
10.21608/aeji.2015.17179
17179
Original Article
Risk of Hepatic Encephalopathy in Diabetic Decompensated Liver Diseased Patients with Post- HCV Liver cirrhosis
Ghada Salem
ghadasalem69@gmail.com
1
Amal Jouda
2
Tropical Medicine Department, Faculty of Medicine, Zagazig University, Egypt
Tropical Medicine Department, Faculty of Medicine, Zagazig University, Egypt
Background and study aim: Hepatic encephalopathy (HE) is a complex and variable neuropsychiatric syndrome that is seen in patients with acute and chronic liver diseases. Diabetes mellitus (DM) is more prevalent in patients with post HCV cirrhosis. Because diabetes mellitus may be associated with delayed gastrointestinal transit and promoting constipation, increasing intestinal bacterial overgrowth and increasing glutaminase activity, we speculated that its presence in patients with HCV related cirrhosis would predispose to and exacerbate hepatic encephalopathy. Patients and Methods: This study included 264 patients with severely decompensated post-HCV cirrhosis, 132 diabetic cirrhotic patients and 132 non-diabetic cirrhotic patients as control group. History is taken for all patients regarding the number of attacks of encephalopathy he experienced in the past three months, the duration of diabetes and the anti-diabetic medication he uses. All patients in the study performed liver function tests, abdominal ultrasound, complete blood count and HBA1c level for diabetic patients as well as psychometric tests for hepatic coma. Results: Diabetic patients had higher frequency of all grades of hepatic encephalopathy mean number of attacks for each patient in the past three months is 1.9±0.3 vs 0.8±0.1 in non-diabetics with unclear precipitating factor in 43% of diabetic patients versus 23% in non-diabetic patients. Patients on oral hypoglycemic drugs represented 14.3% of diabetic patients. Patients with HBA1c >11% were 43% among patients on oral hypoglycemic drugs vs 23% with insulin. Patients on oral hypoglycemic drugs had higher frequency of hepatic coma. The mean number of attacks experienced by each patient rises with increased concentration of HBA1c from 0.8±0.2 at level <7% to 6.4±3for level >11%. The mean number of attacks increased with the duration of diabetes from 1±0.4 for 15 years. Conclusion: The frequency of HE was higher in diabetic patients without other obvious precipitating factor. Patients with uncontrolled diabetes and patients on oral hypoglycemic drugs, and those with longer duration of diabetes seem to have higher risk of developing HE.
https://aeji.journals.ekb.eg/article_17179_1dba956aa193fe28bd496cc9ee542442.pdf
Diabetes mellitus
hepatic encephalopathy
HCV
HbA1c
eng
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
Afro-Egyptian Journal of Infectious and Endemic Diseases
2090-7613
2090-7184
2015-03-19
5
1
40
50
10.21608/aeji.2015.17180
17180
Original Article
Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar Carcinoma
Mohamed El Khashab
sohaesmat@hotmail.com
1
Soha Khorshed
2
Mostafa Toam
3
Hanaa Abdelmoety
4
Shereen Awad
5
Tropical Medicine Department , Faculty of Medicine, Zagazig University, Egypt
Tropical Medicine Department , Faculty of Medicine, Zagazig University, Egypt
Radiotherapy Department , Faculty of Medicine, Zagazig University, Egypt
Clinical Pathology Department , Faculty of Medicine, Zagazig University, Egypt
Family Medicine Department , Faculty of Medicine, Zagazig University, Egypt
Background and study aim : Hepato-cellular carcinoma (HCC) is common all over the world. Most HCC are diagnosed at an advanced stage . We aimed to detect the serum Golgi protein (GP 73) in patients with cirrhosis and HCC as non-invasive marker for diagnosis and prognosis of HCC. Patients and Methods: This study was conducted on 81 subjects: They were divided into 3 groups : 27 patients with HCC, 27 patients with liver cirrhosis and 27 healthy control subjects. Serum alphaphetoprotein (AFP) and GP 73 were estimated by ELISA. In addition, GP 73 was remasured after therapy in patients with HCC who were treated by percutaneous ethanol injection. Results: GP 73 was elevated in patients with HCC and liver cirrhosis ; serum level was high in HCC patients (p<0.01) when compared with the other studied groups. GP 73 had sensitivity of 81.4% and specificity of 100% at a cut-off value 4.12 ng/ml with area under the receiver operator characteristics (AUC) of 0.964 when compared with AFP that showed a sensitivity 77.7% , specificity 85.1% at a cut-off value >200 and (AUC) 0.774. when AFP was combined with GP73 for the diagnosis of HCC, sensitivity and specificity were increased to 87.6% and 100% respectively. At six week after ethanol injection, a significant decrease in GP73 occurred. Conclusion: Serum GP 73 can be used as a useful biomarker to confirm the diagnosis of HCC especially if combined with AFP and GP73 had promising prognostic value as it decreased after the treatment of HCC and is correlated to tumor size
https://aeji.journals.ekb.eg/article_17180_94214a114bd9d247e6731c973082d5b1.pdf
HCC
GP73
AFP
eng
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
Afro-Egyptian Journal of Infectious and Endemic Diseases
2090-7613
2090-7184
2015-03-20
5
1
51
51
10.21608/aeji.2015.17181
17181
Video case: Multiple sessile gastric polyposis in ulcerative colitis patient
Tarek Zaher
tareqzaher@gmail.com
1
General Medicine
43 years old man presented with epigastric pain and bleeding per rectum. Upper gastrointestinal endoscopy (Video1) revealed multiple sessile gastric polyposis. Histopathological examination of one polyp revealed H. pylori related inflammation . Colonoscopy (Video2) detected ulcerations and hyperemic inflammations of the rectal and sigmoid mucosa. Histopathological examination of colonic and rectal biopsies revealed ulcerative colitis with mild activity .Finally the question is: Is there any relation between gastric and colonic lesions?
https://aeji.journals.ekb.eg/article_17181_d9862559de5d9cc792aa42fb290a40ab.pdf
eng
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
Afro-Egyptian Journal of Infectious and Endemic Diseases
2090-7613
2090-7184
2015-03-01
5
1
52
52
10.21608/aeji.2015.17182
17182
Image Case:Hepatic Hydatid Cyst ; an Incidental Finding in Patient with Blunt Abdominal Trauma
Emad Emara
1
Sameh Saber
2
Wael Mansy
3
Radiodiagnosis Department, Faculty of Medicine, Zagazig University, Egypt
Radiodiagnosis Department, Faculty of Medicine, Zagazig University, Egypt
General Surgery Department,Faculty of Medicine, Zagazig University, Egypt
We reported a 13 years old male with blunt abdominal trauma presented with right upper quadrant pain at Emergency Department, Zagazig University Hospitals. It was diagnosed as hepatic hematoma and treated conservatively. Two weeks later, on abdominal ultrasound examination the liver showed large left lobe cyst with separated tissues and daughter cyst inside the sand consistent with Hydatid cyst (Figure 1), the case was further studied by abdominal CT that showed thick walled cyst (Figure 2) and confirmatory MRI was done (Figure 3).
https://aeji.journals.ekb.eg/article_17182_565c351597c7917ac9877a1e17b7eeb1.pdf