Monocyte Chemoattractant Protein-1 as a Diagnostic Marker for Detection of Hepatocellular Carcinoma in Egypt
Sherif
Galal
Departments of Tropical Medicine, Faculty of Medicine, Zagazig University, Egypt
author
Nermin
Raafat
Departments of Medical Biochemistry, Faculty of Medicine, Zagazig University, Egypt.
author
text
article
2016
eng
Background and study aim: Hepatocellullar carcinoma (HCC) is usually diagnosed at advanced stage resulting in limited therapeutic options and poor prognosis. The role of alpha-fetoprotein (AFP) in the diagnosis of HCC is controversial. Here, we investigated the role of Monocyte Chemoattractant Protein -1 (MCP-1), a serum biomarker, alone or in combination with AFP for detection of HCC. Patients and Methods: 116 patients with liver cirrhosis were included. The patients were divided into 2 groups: HCC group included 58 patients with HCC and non-HCC patients as a control group included 58 patients with no evidence of hepatic focal masses. Routine laboratory investigations, AFP, MCP-1, pelvi-abdominal ultrasonography (US) and triphasic computed tomography (CT) scan were performed in all patients. Results: It was found that MCP-1 at a cut-off value >0.390 ng/ml has a sensitivity of 75.8% and specificity of 88.3% with AUROC 0.916; But AFP at a cut-off value >20 ng/ml has a sensitivity of 86.5% and specificity of 96.4% with AUROC 0.924, while combined (AFP+ MCP-1) at a cut-off value >23.390 ng/ml has a higher sensitivity (96.5%) specificity of 100% with AUROC 0.995. Conclusion: Monocyte Chemoattractant Protein-1 (MCP-1) can be identified as an adjuvant biomarker for HCC detection. Combined (AFP+MCP-1) showed higher diagnostic ability than MCP-1 alone or AFP alone in HCC detection.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
2
no.
2016
75
81
https://aeji.journals.ekb.eg/article_9670_9361a808faa047f136e5ff264412d156.pdf
dx.doi.org/10.21608/aeji.2016.9670
Comparative Study of MELD Score and Glasgow Coma Scale in Patients with Hepatic Encephalopathy
Samir
Kabil
Hepatology, Gastroenterology and Infectious Diseases Department, Faculty of Medicine,
Benha University,Benha ,Egypt.
author
Magdy
Gad
Hepatology, Gastroenterology and Infectious Diseases Department, Faculty of Medicine,
Benha University,Benha ,Egypt.
author
Naglaa
El-Toukhy
Hepatology, Gastroenterology and Infectious Diseases Department, Faculty of Medicine,
Benha University,Benha ,Egypt.
author
Nahed
Abdel-Moteleb
Hepatology, Gastroenterology and Infectious Diseases Department, Faculty of Medicine,
Benha University,Benha ,Egypt.
author
text
article
2016
eng
Background and study aim: Hepatic encephalopathy occurs in approximately 30-45% of patients with cirrhosis and 10–50% of patients with transjugular intrahepatic Porto systemic shunt, while minimal hepatic encephalopathy affects approximately 20–60% of patients with liver disease. There are multiple prognostic scores that predict the mortality from chronic liver disease, of which the Child-Pugh score and the Model for End-stage Liver Disease (MELD) score are the most commonly used. The most widely used scale used to evaluate HE is the West-Haven (WH) scale, with scores ranging from 0 to 4. This study is designed to compare Glasgow coma scale to West-Haven scale in prediction of prognosis and survival of patients with hepatic encephalopathy. Patients and Methods: This study was conducted on 100 patients with liver cirrhosis and overt Hepatic Encephalopathy admitted to The Department of Hepatology, Gastroenterology and Infectious Diseases of Mansoura Health Insurance Hospital, divided into four groups according to the grade of encephalopathy by West-Haven Criteria. Results: There was no difference in prediction of survival among the studied patients assessed by GCS, MELD score, uMELD score and Child score (all had the same results). Conclusion: Glasgow Coma Scale can be a prognostic tool for morbidity and mortality, as well as, follow-up in patients with HE
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
2
no.
2016
82
93
https://aeji.journals.ekb.eg/article_9673_b3da941c62eaa03e2e52df4aa4bda255.pdf
dx.doi.org/10.21608/aeji.2016.9673
Dermatological Manifestations of Pegylated Interferon alfa2a and Ribavirin Combination Therapy in Chronic HCV Patients
Mohamed
Khater
Department of Dermatology and Andrology, Faculty of Medicine, Zagazig University, Egypt
author
Fathia
Khattab
Department of Dermatology and Andrology, Faculty of Medicine, Zagazig University, Egypt
author
Mohamed
Refaey
Tropical Medicine Department, Faculty of Medicine, Zagazig University, Egypt
author
text
article
2016
eng
Background and study aim: Dermatological adverse events are an existing concern during treatment of chronic hepatitis C virus infection. Pegylated Interferon (peg –IFN- alfa2a) and ribavirin combination therapy is associated with well-characterized dermatological lesions tending towards a uniform entity of dermatitis. A prospective observational study was designed to evaluate the frequency and clinical pattern of cutaneous side effects in a cohort of patients receiving combination therapy of (pegylated interferon alfa2a) and ribavirin for chronic hepatitis C infection. Patients and Methods: This study was carried out at Alahrar Center for treating chronic hepatitis C patients which is one of the centers of the national committee for treating chronic hepatitis C patients (HCV), Zagazig, Sharkia governorate, Egypt over a period of one year starting from January, 2014 to December 2014. A cohort of 116 consecutive, HCV-positive patients to be treated with pegylated interferon alfa2a and ribavirin with standard doses, were prospectively enrolled. After taking an informed consent, detailed history and cutaneous examination, before treatment and then monthly follow up for one year(during the course of treatment) were performed and recorded .All patients were subjected to throughout, routine laboratory investigations before enrollment including, CBC, random blood sugar, complete liver and renal function tests, TSH, Alfa fetoprotein, antibilharzial Ab titre, ANA, P.T, INR, quantitative PCR for HCV-RNA, pregnancy test was performed for the ladies. Results: 113/116 patients (97%) experienced 1 or more cutaneous side effects. The most frequent was hair loss and occurred in 69 cases (Session [CurrentTestPartTermID]%). Pigmented Oral lichen planus was noted in 50 cases (43%) and generalized pigmentation in 32 (27%). Hypertrichosis of eyelashes (trichomegaly) and eyebrows (synophyrs) was observed in 42 (36%) and 40 (34%) cases respectively. Pruritus occurred in 50 cases (43%), aphthous stomatitis was observed in 33 cases (38%), 19 patients (22%) either developed or had worsening of melasma and 23 (27%) developed urticaria. Brittle nails (10 cases), cheilitis (8 cases), glossitis (3 cases), actinic lichen planus (9 cases), greying of hair (3 cases), discoloration of moustache hair (1 case), and photo-sensitivity (3 cases) were also observed. Preexisting psoriasis (8 cases), and lichen planus (5 cases) aggravated. Eruptive seborrhoeic keratosis was reported in 1 case. Conclusion: 113/116 patients (97%) experienced 1 or more cutaneous side effects. The most frequent was hair loss and occurred in 69 cases (Session [CurrentTestPartTermID]%). Pigmented Oral lichen planus was noted in 50 cases (43%) and generalized pigmentation in 32 (27%). Hypertrichosis of eyelashes (trichomegaly) and eyebrows (synophyrs) was observed in 42 (36%) and 40 (34%) cases respectively. Pruritus occurred in 50 cases (43%), aphthous stomatitis was observed in 33 cases (38%), 19 patients (22%) either developed or had worsening of melasma and 23 (27%) developed urticaria. Brittle nails (10 cases), cheilitis (8 cases), glossitis (3 cases), actinic lichen planus (9 cases), greying of hair (3 cases), discoloration of moustache hair (1 case), and photosensitivity (3 cases) were also observed. Preexisting psoriasis (8 cases), and lichen planus (5 case) aggravated. Eruptive seborrhoeic keratosis was rep case).
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
2
no.
2016
94
102
https://aeji.journals.ekb.eg/article_9675_7c62054c8bdb9ded437123f1292263d1.pdf
dx.doi.org/10.21608/aeji.2016.9675
Interferon-Gamma Inducible Protein-10(IP10) as a Predictor of Early Virologic Response in Chronic Hepatitis C Infected Egyptian Patients Stratified for the Interleukin-28B rs12979860 Genotyp
Mohamed AM
Bahgat
Departments of Tropical Medicine, Faculty of Medicine, Zagazig University
author
Walid
Abdeldayem
Departments of Tropical Medicine
, Faculty of Medicine, Zagazig University
author
Heba
F Pasha
Departments of Biochemistry
, Faculty of Medicine, Zagazig University
author
Hala
Hussein
Departments of Biochemistry
, Faculty of Medicine, Zagazig University
author
Ibrahim
Ibrahim
Departments of Biochemistry
, Faculty of Medicine, Zagazig University
author
Ahmed
Abdelmoaty
Departments of Biochemistry
, Faculty of Medicine, Zagazig University
author
text
article
2016
eng
Background and study aim: Single nucleotide polymorphisms near Interleukin 28B are strongly associated with favourable treatment response of chronic hepatitis C such as, the homozygous CC at markers129798Session [CurrentTestPartID]. Interferon-Gamma Inducible Protein-10 (IP-10) can be produced by a variety of cells, including hepatocytes. Pre-treatment plasma levels of IP10 are elevated in patients chronically infected with hepatitis C virus of genotypes 1 or 4 who do not achieve early virological response(EVR)to treatment. The aim of this study was to evaluate the rule of adding IP-10 to IL28B rs129798Session [CurrentTestPartID] Genotype in predicting EVR .
Patients and methods: The study enrolled 78 naïve chronic HCV patients who have criteria that met the pegylated interferon plus ribavirin (pegIFN-RBV) treatment for chronic hepatitis C virus (HCV) .IP-10 assay and single nucleotide polymorphisms of the IL28B genotype were performed.
Results: Patients with EVR was younger than those without EVR with statistically significant different. Patients with EVR had less elevated liver enzyme ,low viral load and fasting blood sugar than those without EVR with statistically significant difference. 100% patients with out early virological response had A2 activity while 31.7% only of patients with EVR had A2 activity with statistically significant difference .Patients with EVR showed lower level of IP10 and 81.7% of them had CC allele genotype with statistically significant difference when compared to patients without EVR. Patients with CC genotype were associated with lower level of IP10, ALT, AST and also low viral load. Patients with low level of IP10 had lower levels of liver enzyme. Cut off level of IP 10 was<Session [CurrentTestPartID]5 pico gram/ml; at this cut off value sensitivity was=100%, specificity was= 96.7% and area under the curve (AUC)= 0.99.
Conclusion: IP10 level was lower among responder group. IL28 genotype CC was significantly higher in responders when compared with non responders. .Patients with CC genotype were associated with lower level of IP10 and liver enzymes. Patients with CC genotype were associated also with low viral load.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
2
no.
2016
103
111
https://aeji.journals.ekb.eg/article_9948_55a6a92b8ee44b818b0dc7afbff2aba6.pdf
dx.doi.org/10.21608/aeji.2016.9948
Gut Microbiota and Type 2 Diabetes Mellitus : What is The Link ?
Nesma
Ibrahim
Internal Medicine Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
author
text
article
2016
eng
The rapid increase of cases of type 2 diabetes mellitus (T2DM) in the past decades has made it a widespread metabolic disorder. In addition to well-established risk factors for T2DM, including genetic predisposition, poor physical activity, fetal programming and obesity, an altered configuration of the microbial community in our gut – the microbiota – has emerged as a new candidate that may be linked to T2DM. The aim of this review is to focus the light on the role of gut microbiota as a novel key organ involved in metabolism, and discussing the putative mechanisms linking gut microbiota and T2DM, as well as the therapeutic perspective of intestinal microbiota modulation for T2DM.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
2
no.
2016
112
119
https://aeji.journals.ekb.eg/article_9950_e39181d3625bf178121c6ced2676a534.pdf
dx.doi.org/10.21608/aeji.2018.9950
Image Case: Visualization of moving living adult worm (Entrobious) during total colonoscopy
Mohamed
Refaey
Tropical Medicine Department, Faculty of Medicine, Zagazig University, Egypt.
author
Tarik
Zaher
Tropical Medicine Department, Faculty of Medicine, Zagazig University, Egypt.
author
text
article
2016
eng
Visualization of moving living adult worm(Entrobious) during total colonoscopy for 42 yrs.old male complaining of chronic diarrhea with abdominal pain.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
2
no.
2016
120
120
https://aeji.journals.ekb.eg/article_9952_41861ea865612f42fe6bf86f4c4b0e55.pdf
dx.doi.org/10.21608/aeji.2016.9952