Impact of Helicobacter pylori Eradication on Absolute Telomere Length in Gastric Mucosa
Maha
. Omar
Department of Hepatology, Gastroenterology and Infectious Diseases, Faculty of Medicine, Benha University, Egypt
author
Abeer
Aboelazm
Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt
author
Reem
Abd El-Glil
Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt
author
text
article
2016
eng
Background and study aim: This study proposed to assess the relation between absolute telomere lengths (TLs) in gastric mucosa and Helicobacter pylori (H. pylori) infection and study the impact of (H. pylori) eradication therapy on TLs. Patients and Methods: This study was conducted on (42) subjects divided into two groups, group I: included (17) H. pylori negative cases served as control group, group II: included (25) H. pylori positive patients. Absolute TLs was measured in base pairs (bp) in gastric mucosa and analyzed by real time polymerase chain reaction (RT-PCR) in all groups and re-evaluated in H. pylori positive patients 4 weeks after eradication therapy. Results: Prior to eradication therapy, there was highly significant shortening (P<0.001) in TLs (bp) in gastric mucosa of H. pylori positive patients compared to H. pylori negative controls. While there was highly significant elongation was observed after H. pylori eradication therapy in H. pylori positive patients (P < 0.001). There was significant negative correlation between TLs and ages of patients (P<0.001) while there was no significant relation between TLs and sex (P= 0.5) before and after eradication therapy. Conclusion: H. pylori positive patients had significantly shorter TLs than H. pylori negative controls. TLs were increased after H. pylori eradication therapy in H. pylori positive cases. This finding may indicate the importance of H. pylorieradication to avoid the development of gastric cancer by its effect on TLs.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
3
no.
2016
121
127
https://aeji.journals.ekb.eg/article_9954_216936c3cf806aec85ec7077ccac690c.pdf
dx.doi.org/10.21608/aeji.2016.9954
Relationship between Plasma Concentrations of Interleukin-10 and Thrombocytopenia in Chickenpox Patients
Mariam
Al-Fadhli
Department of Medicine, Infectious Disease Hospital, Ministry of Health, Kuwait
author
Mohammad
Saraya
Department of Tropical Medicine, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
author
text
article
2016
eng
Background and study aim: Chickenpox is highly infectious, self-limiting disease, caused by varicella-zoster virus. Thrombocytopenia frequently complicates chickenpox. This study was conducted to assess the correlation between serum interleukin 10 levels and thrombocytopenia in adult chickenpox patients.
Patients and Methods: Fifty patients of chickenpox complicated by thrombocytopenia were included in this study as a patient group. Fifty patients of chickenpox with normal platelet of comparable age, race and gender were identified as a control group. All patients were diagnosed by clinical picture of chickenpox and by presence of varicella - zoster virus immunoglobulin M (VZV IgM) in the patient blood. Estimation of complete blood count, kidney function tests, liver function tests, fasting blood sugar, and levels of cytokines IL-10 and TNF-α were done in both groups on the day of admission and day of discharge.
Results: At time of admission, IL-10 levels were significantly higher in patients group than in control group, while TNF-α level were not significantly different between the two groups. At time of discharge, we observed a significant decline in IL-10 levels in the patients group as compared with time of admission. A negative correlation between IL-10 levels and platelet count was observed while no correlation was found between TNF-α and platelet count.
Conclusion: IL-10 level could play an important role in development of thrombocytopenia in patients with chickenpox.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
3
no.
2016
128
133
https://aeji.journals.ekb.eg/article_9956_97bcc0dbccf2597298342252d2169b47.pdf
dx.doi.org/10.21608/aeji.2016.9956
Frequency of Cholelithiasis in Patients with Chronic Liver Disease: A Hospital-Based Study
Mona
Abdelmaksoud
Tropical Medicine department, Faculty of Medicine, Zagazig University, Egypt
author
Mostafa
El-Shamy
Tropical Medicine department, Faculty of Medicine, Zagazig University, Egypt
author
Hala
Hussein
Tropical Medicine department, Faculty of Medicine, Zagazig University, Egypt
author
Bihery
Ahmed
Tropical Medicine department, Faculty of Medicine, Zagazig University, Egypt
author
Hussein
Ahmed
Medical Research Group of Egypt ,Faculty of Medicine, Zagazig University, Zagazig, Egypt
author
Hoda
El-Hady
Internal Medicine Department, Faculty of Medicine, Zagazig University, Egypt
author
text
article
2016
eng
Background and study aim: Liver Cirrhosis is a strong and a common known risk factor for Cholelithiasis. Cholelithiasis is a multifactorial disease, based on a complex interaction of environmental and genetic factors. The primary aim of this study is to determine the frequency of cholelithiasis in chronic liver disease (CLD) patients admitted to Zagazig university hospitals. The secondary aim is to determine the risk factors and their association with the underlying etiology and severity of liver disease. Patients and Methods: We conducted a hospital based study including 131 patients with chronic liver disease based on clinical, laboratory and Ultrasonographic findings. Demographic, clinical and etiological data were recorded, using a pre-coded questionnaire. A number of laboratory tests as fasting plasma glucose, total cholesterol, triglyceride, aspartate aminotransferase (AST), alanine amino-transferase (ALT), alkaline phosphatase (ALP), hepatitis B surface antigen (HBsAg), and antibody to hepatitis C virus (HCV-Ab) were analyzed. Results: The number of registered cases was 131 with age (52.9±11.7).There were 55 (42%) males and 76 (58%) females. Hepatitis C (HCV) was present in 101 (77%) cases. The prevalence of cholelithiasis was 50.4%% (66 of 131 patients). Most of cholelithiasis patients presented with child C stage (68.2%), followed by child B (21.2%) and the least one was Child A. Hepatitis C (10.6%) was found to be associated with cholelithiasis (75.8%), followed by hepatitis B (13.6%). Auto-immune disease, diabetes mellitus, contra-ceptive pills and obesity are considered risk factors for cholelithiasis. Conclusion: Cholelithiasis tends to occur more frequently in patients with decompensated CLD. The higher incidence of cholelithiasis in CLD appears to be associated with HCV infection. This is an important parameter to be considered in a country with high prevalence of HCV as Egypt.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
3
no.
2016
134
141
https://aeji.journals.ekb.eg/article_9958_6698067ac0b19fc581f890873e4d4cd6.pdf
dx.doi.org/10.21608/aeji.2016.9958
A molecular Approach for Evaluation of Experimental Trials of Anti Schistosomal Vaccination in Murine Models
Samia
Etewa
Medical Parasitology Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.
author
Mohamed
Abdel Hady
Medical Parasitology Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.
author
Ashraf
Metwally
Medical Parasitology Departments, Faculty of Medicine
Zagazig University, Zagazig, Egypt.
author
Somia .
Abd Allah
Biochemistry Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.
author
Sally
Shalaby
Biochemistry Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.
author
Amal
El-Shal
Biochemistry Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt
author
Mahmoud
El Shafey
Clinical Pathology Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.
author
Howayda
Moawad
Medical Parasitology Department, Faculty of Medicine
Zagazig University, Zagazig, Egypt.
author
text
article
2016
eng
Background and study aim: Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believed that the best long term strategy to control schistosomiasis is through immunization with anti-schistosomiasis vaccines. This study aims at assessment of the efficacy of different potential anti-schistosomal vaccines (as crude soluble egg antigens (SEA), soluble worm antigen preparation (SWAP) and combined SEA & SWAP) by parasitological and molecular studies in experimental murine models.
Materials and Methods: Sixty male laboratory bred Swiss Albino mice were used and divided into six groups; control normal (G1), control infected by ± 80 cercariae by S.C. route (G2), Freund’s adjuvant (adj.) received then infected (G3), SEA+adj. received then infected (G4), SWAP+ adj. received then infected (G5) and combined (SEA+SWAP) + adj. received then infected (G6). A schedule of sensitization, immunization and schistosomiasis challenge were followed and performed on different mice groups. Mice were euthanized 10 weeks post-infection. Potential vaccine efficacy was investigated by parasitological and molecular studies including egg count/gram stool using modified Kato thick smear,liver egg load, oogram pattern in the liver and stool PCR to detect S. mansoni egg DNA in stools of studied mice.
Results: The combined (SEA+SWAP) vaccine caused the highest significant reduction in the fecal egg count followed by SWAP then SEA antigens. On the other hand, the highest percentage reduction in eggs/gram liver tissue was attributed to the combined (SEA+SWAP) followed by SEA then SWAP antigens. Regarding oogram results, the combined (SEA+ SWAP) antigens were more efficient in increasing the number of dead ova with highly significant reduction in the number of mature & immature ova, followed by SEA then SWAP antigens. The lowest percentage of S. mansoni egg DNA detected by PCR in stool samples was encountered with the combined (SEA+ SWAP), followed by SEA then SWAP antigens.
Conclusion:The parasitological and PCR-based assessment studies denoted that the combined (SEA+SWAP) vaccine candidate was the most effective in protection against schistosomiasis challenge. The results of parasitological and molecular studies were nearly similar but the molecular study was more sensitive, definite and accurate.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
3
no.
2016
142
151
https://aeji.journals.ekb.eg/article_9960_5ff7023dd570c829a0ebaac273c9bff3.pdf
dx.doi.org/10.21608/aeji.2016.9960
Hydatidosis in Morocco: Review of literature and epidemiology
Zineb
Tlamcani
Department of Parasitology, Faculty of Medicine and Pharmacy of Fes,
University of Sidi Mohammed Ben Abdellah ,Fes, Morocco,
author
text
article
2016
eng
Hydatidosis or hydatid cyst is a prevalent zoonosis all over the world due to larval forms of the tapeworm of the genus Echinococcus. Echinococcus granulosus is the most frequent form of echinococcal infection in humans. The disease generally results from an oral ingestion of the parasite ova eliminated with feces of dogs leading to the emergence of hydatid cysts. Hydatidosis is a health problem that may remain asymptomatic for several years. This disease is endemic in Morocco and is recorded to be a serious problem that requires great caution.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Zagazig University, Faculty of Medicine, Endemic and Tropical Medicine Department
2090-7613
6
v.
3
no.
2016
152
155
https://aeji.journals.ekb.eg/article_9962_ff1ffb18c0a1b0c0dfa109f35195a8fe.pdf
dx.doi.org/10.21608/aeji.2016.9962